Microscopic Study of Crystal Growth in Cryo-preservation Solutions
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چکیده
Ice formation inside or outside cells during cryo-preservation has been proved to be the main cause for cryo-injury to cells. A high voltage DC electric field combined with a cryomicroscopic stage was used in this study. Dendritic ice crystals became asymmetric with the addition of electric field. DMSO and NaCl solutions were tested under different electric field strengths ranging from 83kV/m to 320kV/m. The shape change of ice crystals in the NaCl solution seemed more pronounced, because the NaCl solution was an ionic solution.
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تاریخ انتشار 2001